Energy Flow and Nutrient Cycles, Grade 10: Food Chains, Pyramids and Cycles

This page covers the third part of the Grade 10 topic Biosphere to ecosystems: how energy flows through an ecosystem, food chains, food webs and ecological pyramids, and the water, oxygen, carbon and nitrogen cycles. It ends with two short classroom demonstrations.
| Subject | Life Sciences |
|---|---|
| Grade and term | Grade 10, Term 3 |
| Topic | Biosphere to ecosystems, part 3 of 3: energy flow and nutrient cycles |
| Practicals | Classroom demonstrations: transpiration in a plastic bag, and root nodules on a legume. The fieldwork for this topic is on the study an ecosystem page |
| Time | Transpiration: 10 minutes to set up, results after 1 to 2 hours in the sun. Root nodules: about 20 minutes |
Trophic levels
A trophic level is a feeding level: the position an organism takes in a food chain, counted from the producers.
| Trophic level | Feeds on | Examples |
|---|---|---|
| Producers, first level | Make their own food by photosynthesis | Grass, trees, algae |
| Primary consumers, second level | Producers. These are herbivores | Grasshopper, zebra, impala |
| Secondary consumers, third level | Herbivores. These are carnivores | Lizard, jackal, lion |
| Tertiary consumers, fourth level | Other carnivores | A hawk that eats lizards |
Omnivores, such as baboons and people, eat both plants and animals, so they feed at more than one level. Decomposers, mainly bacteria and fungi, get their energy by breaking down dead organisms and waste from every level.
Food chains and food webs
A food chain shows who eats whom, for example:
grass → zebra → lion
The arrows show the direction in which energy flows, from the organism that is eaten to the organism that eats it. Most animals eat more than one kind of food and are eaten by more than one predator, so in a real ecosystem the chains link up into a food web. A food web gives a truer picture of an ecosystem than a single chain does.

Energy flow
Almost all the energy in an ecosystem comes from the Sun. Producers capture it by photosynthesis and store it in food. When one organism eats another, only about 10% of the energy at one trophic level is passed on to the next. The rest is lost:
- Most is used in respiration, for moving and staying alive, and is lost as heat.
- Some parts are not eaten, such as roots, bark, bones and fur.
- Some food is not digested and leaves the body in the faeces.
- Organisms that die without being eaten pass their energy to the decomposers.

| Trophic level | Energy, using the 10% rule |
|---|---|
| Producers | 10 000 kJ |
| Primary consumers | 1 000 kJ |
| Secondary consumers | 100 kJ |
| Tertiary consumers | 10 kJ |
This is why food chains seldom have more than four or five links: there is too little energy left at the top to support another level. It is also why a field of maize feeds more people when they eat the maize than when it is fed to cattle and they eat the beef.
Class activity: a field of grass captures 100 000 kJ of energy from the Sun. Ask learners to work out how much reaches a grasshopper, a lizard that eats grasshoppers, and a hawk that eats lizards. The answers are 10 000 kJ, 1 000 kJ and 100 kJ.
Ecological pyramids
An ecological pyramid shows the trophic levels as bars stacked on top of each other, with the producers at the bottom. There are three kinds, each drawn from a different measurement.
| Pyramid | What each bar shows |
|---|---|
| Numbers | The number of organisms at each level |
| Biomass | The total mass of the organisms at each level |
| Energy | The energy passing through each level in a given time |
A pyramid of biomass is not always pyramid-shaped. In parts of the open sea, the animals and bacteria in the plankton can outweigh the tiny algae they depend on, so the pyramid is upside down. A pyramid of energy is never upside down, because energy is lost at every level, so each bar is smaller than the one below it.
Nutrient cycles
Energy flows through an ecosystem in one direction and is lost as heat. Matter is different: the Earth gets almost no new supply of it, so the same water, carbon, oxygen and nitrogen are used over and over. They move between living things and the environment in cycles.
The water cycle

| Process | What happens |
|---|---|
| Evaporation | The Sun heats water in the sea, rivers, dams and soil, and it becomes water vapour in the air |
| Transpiration | Plants take up water through their roots and lose water vapour from their leaves |
| Condensation | Water vapour cools and forms tiny droplets, which make clouds |
| Precipitation | Water falls back to the ground as rain, hail or snow |
| Run-off and infiltration | Water flows over the ground into rivers and the sea, or soaks into the soil and becomes groundwater |
Animals also return water to the environment when they breathe out, sweat and pass urine.
The oxygen cycle

Producers release oxygen into the air during photosynthesis. Plants, animals and decomposers take oxygen in for respiration, and burning fuels also uses oxygen up. Photosynthesis replaces the oxygen that respiration and burning remove.
The carbon cycle

| Carbon leaves the air by | Carbon returns to the air by |
|---|---|
| Photosynthesis: producers take in carbon dioxide and make it into food | Respiration by plants, animals and decomposers |
| Decomposition of dead organisms and waste | |
| Burning of fossil fuels such as coal, oil and gas, and of wood |
Carbon passes along food chains as animals eat plants and each other. Over millions of years, buried remains of organisms can become coal, oil and gas, which store carbon until they are burnt.
The nitrogen cycle
Air is about 78% nitrogen, but plants and animals cannot use nitrogen gas directly. They need nitrogen to make proteins, and plants take it up from the soil as nitrates. Bacteria do most of the work of the cycle.

| Step | What happens |
|---|---|
| Nitrogen fixation | Nitrogen-fixing bacteria turn nitrogen gas into compounds plants can use. Some live free in the soil, and some live in root nodules on legumes such as beans, peas and clover. Lightning also fixes a small amount |
| Absorption | Plants absorb nitrates through their roots and use them to make proteins. Animals get their nitrogen by eating plants or other animals |
| Decomposition | Decomposers break down dead organisms, faeces and urine, and release ammonium compounds into the soil |
| Nitrification | Nitrifying bacteria turn ammonium compounds into nitrites and then into nitrates |
| Denitrification | Denitrifying bacteria, mainly in waterlogged soil, turn nitrates back into nitrogen gas, which returns to the air |
Classroom demonstration 1: transpiration in a plastic bag
Aim
To show that plants give off water vapour from their leaves, which is how plants take part in the water cycle.
What you need
- A leafy potted plant, such as a bean plant
- A second pot of the same soil with no plant, and a bare stick about the height of the plant pushed into it
- 2 clear plastic bags, about 20 × 30 cm
- 2 elastic bands or pieces of string
- Water and a sunny windowsill or bench
Method
- Water both pots the evening before.
- Slip a plastic bag over the leaves of the plant and tie it gently around the stem just above the soil, so the soil is outside the bag and cannot add water to it.
- Slip the second bag over the stick and tie it around the stick just above the soil in the same way. This is the control.
- Stand both pots side by side in the sun.
- Look at both bags after 1 hour and again after 2 hours.
What you should see
| Bag | After 1 to 2 hours |
|---|---|
| Over the plant | Misty inside, with water droplets on the plastic |
| Over the stick | Dry |
Why it happens
The plant takes up water through its roots and carries it up the stem to the leaves. Water evaporates from the leaf cells and the vapour leaves through tiny pores called stomata. Inside the bag the vapour cannot escape, so it condenses on the plastic. The dry control bag shows that the water came from the plant, not from the soil, the air or the bag. Out in the open the vapour joins the air, so plants return a large amount of water to the atmosphere.
Safety
- Keep plastic bags away from small children.
- Wash your hands after handling soil.
Classroom demonstration 2: root nodules on a legume
Aim
To look at the root nodules where nitrogen-fixing bacteria live.
What you need
- A bean or pea plant at least 6 weeks old grown in garden soil, or a clover plant from the school lawn
- A garden trowel or spade
- A bucket of water
- A white tile and a sharp knife, used by the teacher
- A hand lens, if you have one
Method
- Dig up the plant with a wide ball of soil around the roots, so the fine roots are not torn off.
- Soak the roots and soil in the bucket of water for 10 minutes.
- Swirl the roots gently until the soil falls away.
- Look along the roots for small round or finger-shaped lumps.
- The teacher cuts one lump in half on the tile.
- Look at the colour inside.
What you should see
| What you look at | What you see |
|---|---|
| The roots | Small round or finger-shaped lumps a few millimetres long. These are the root nodules |
| Inside a nodule | Pink or red if the bacteria are fixing nitrogen. Young nodules are white or grey inside, and old ones that have stopped fixing nitrogen turn green |
Why it happens
Nitrogen-fixing bacteria called Rhizobium enter the roots of legumes, and the plant grows nodules around them. The bacteria turn nitrogen gas from the air in the soil into compounds the plant uses to make proteins, and the plant gives the bacteria sugars in return. The pink colour comes from a pigment that controls how much oxygen reaches the bacteria. Because legumes add nitrogen compounds to the soil, farmers often grow them in rotation with crops such as maize.
Safety
- Only the teacher uses the knife, cutting on the tile and away from the hand.
- Wash your hands after handling soil.
Free worksheet and marking memo
- Learner worksheet, covering energy flow, pyramids, the nutrient cycles and both demonstrations
- Marking memorandum
Related practicals
- Biosphere to ecosystems, Grade 10, part 1 of this topic: the biosphere, biomes and ecotourism
- Biotic and abiotic factors, Grade 10, part 2 of this topic
- Study an ecosystem, Grade 10, the fieldwork for this topic
- Food chains and food webs, Grade 8
- Photosynthesis and respiration, Grade 8
- Micro-organisms, Grade 11
- The chemistry of life, Grade 10
- Human impact on the environment, Grade 11
- Biodiversity and classification, Grade 7
References
- Bernhard, A. (2010) 'The nitrogen cycle: processes, players, and human impact', Nature Education Knowledge, 3(10), p. 25. Available at: https://www.nature.com/scitable/knowledge/library/the-nitrogen-cycle-processes-players-and-human-15644632/ (Accessed: 5 November 2026).
- Britannica Editors (n.d. a) Biosphere. Encyclopaedia Britannica. Available at: https://www.britannica.com/science/biosphere (Accessed: 5 November 2026).
- Britannica Editors (n.d. b) Food chain. Encyclopaedia Britannica. Available at: https://www.britannica.com/science/food-chain (Accessed: 5 November 2026).
- Britannica Editors (n.d. c) Trophic pyramid. Encyclopaedia Britannica. Available at: https://www.britannica.com/science/trophic-pyramid (Accessed: 5 November 2026).
- Flynn, R. and Idowu, J. (2015) Nitrogen fixation by legumes. Las Cruces: New Mexico State University Cooperative Extension Service. Available at: https://pubs.nmsu.edu/_a/A129.pdf (Accessed: 5 November 2026).
- Gasol, J.M., del Giorgio, P.A. and Duarte, C.M. (1997) 'Biomass distribution in marine planktonic communities', Limnology and Oceanography, 42(6), pp. 1353-1363. Available at: https://doi.org/10.4319/lo.1997.42.6.1353 (Accessed: 5 November 2026).
- National Aeronautics and Space Administration (n.d.) Earth fact sheet. NSSDCA. Available at: https://nssdc.gsfc.nasa.gov/planetary/factsheet/earthfact.html (Accessed: 5 November 2026).
- National Oceanic and Atmospheric Administration (n.d.) Carbon cycle. NOAA Education. Available at: https://www.noaa.gov/education/resource-collections/climate/carbon-cycle (Accessed: 5 November 2026).
- U.S. Geological Survey (2018) Evapotranspiration and the water cycle. Water Science School. Available at: https://www.usgs.gov/special-topics/water-science-school/science/evapotranspiration-and-water-cycle (Accessed: 5 November 2026).
- U.S. Geological Survey (n.d.) The water cycle. Water Science School. Available at: https://www.usgs.gov/special-topics/water-science-school/science/water-cycle (Accessed: 5 November 2026).